• CAMP-test revers_Arcanobacterium haemolyticum
  • CAMP-test revers_Clostridium perfringens
  • CAMP-test revers_Corynebacterium ulcerans
  • CAMP-test_Actinomyces neuii subsp neuii
  • CAMP-test_Listeria monocytogenes & L. ivanovii
  • CAMP-test_Streptococcus agalactiae
  • Catalase
  • Default page for tests.php * PLEASE CHANGE THIS TITLE *
  • Dienes phenomenon test_P. mirabilis
  • Fluorescence_Fusobacterium necrophorum, F. nucleatum and F. species
  • Gram staining
  • Granules_Aggregatibacter actinomycetemcomitans
  • Indole test
  • Lipophylic Corynebacteria
  • Medusa head
  • Methylene Blue stain
  • Motility
  • Motility_Listeria monocytogenes_semi solid media
  • Motility_Proteus mirabilis
  • Nitrocefin DrySlide™
  • Optochin test
  • Oxidase test
  • Pyrase test
  • Satellitism test_Haemophilus influenzae
  • Triple Sugar Iron (TSI) Agar

Pyrase test

  • General

    • Pyrase / BBL™ DrySlide
      Pyrrolidonyl Arylamidase (PYR) test is a rapid test which is used for the presumptive identification of group A beta-hemolytic Streptococci and Enterococci.

      The substrate for the PYR test is L-naphtylamide-ß-naphtylamide wich is hydrolyzed by a specific bacterial enzyme.

      Hydrolysis of the substrate by this enzyme releases free ß-naphtylamide, which is detected by the addition of N,N-dimethylaminocinnamaldehyde.
      This detection reagent couples with the naphthylamide to form a red Schiff base.

      Procedure of PYR test
      The Dryslide contain dried chromogenic substrates for pyrrolidonyl peptidase (PYRase) and beta-gluconidase.
      1. Applie only a few colonies to the test strip
      2. After 2 minutes
      3) Positive: S. pyogenes, E. faecalis and E. faecium

      Negative: No color change or a blue color due to a positive indole reaction.
      Group B Streptococci (Streptococcus agalactiae), Streptococcus mitis, S. bovis, S. equinus, S. milleri.

      Note: A pale pink reaction (weak) is considered negative.

  • History

  • Related

  • References

    • MicrobeOnline